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Comentario: 
A headspace-gasoline chromatography-tandem mass spectrometry 
(HS-GC-MS/MS) method for the trace measurement of perfluorocarbon compounds (PFCs) in blood was developed.
Attributable to oxygen carrying capabilities of PFCs, application to doping and sports misuse is speculated.
This study was due to this fact prolonged to carry out validation methods for F-tert-butylcyclohexane (Oxycyte(®)), perfluoro(methyldecalin) (PFMD) and perfluorodecalin (PFD).
The restrict of detection of these compounds was established 
and located to be 1.2 µg/mL blood for F-tert-butylcyclohexane, 4.9 µg/mL 
blood for PFMD and 9.6 µg/mL blood for PFD.
The limit of quantification was assumed to be 12 µg/mL blood (F-tert-butylcyclohexane), 
forty eight µg/mL blood (PFMD) and 96 µg/mL blood (PFD).
HS-GC-MS/MS technique permits detection from 1000 to 10,000 instances decrease 
than the estimated required dose to ensure a biological impact for the investigated 
PFCs. Thus, this technique could possibly be used to identify a PFC misuse a number of hours, perhaps 
days, after the injection or the sporting event.
Clinical trials with these compounds are nonetheless required to evaluate the validation parameters with the calculated estimations.


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